HIST1H4A (Ab-31) AntibodyСпецификацияОбъем | 50 мкл | Синонимы | Histone H4, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4, H4/A H4FA, H4/I H4FI, H4/G H4FG, H4/B H4FB, H4/J H4FJ, H4/C H4FC, H4/H H4FH, H4/M H4FM, H4/E H4FE, H4/D H4FD, H4/K H4FK, H4/N H4F2 H4FN HIST2H4, H4/O H4FO, | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P62805 | Иммуноген | Peptide sequence around site of propionyl-Lys (31) derived from Human Histone H4 | Источник | Rabbit | Видовая специфичность | Human, Mouse | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:2000, IHC:1:1-1:100 | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antigen Affinity Purified | Абревеатура | Histone H4 | Области исследований | Epigenetics and Nuclear Signaling | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: HIST1H4A antibody at 1.24µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
| IHC image of CSB-PA010429OA31npropHU diluted at 1:15 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Информация для заказаОбласть использования: | Производство: | Cusabio | Метод: | Антитела | Объем: | 50 мкл | Кат. номер: | CSB-PA010429OA31npropHU | Цена (с НДС 20%): | по запросу | В корзину | Наименование: HIST1H4A (Ab-31) Antibody. Примечание: дополнительная информация (на английском языке). |
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