| Объем | 100 мкл |
| Синонимы | H2A.X, H2AFX, H2a/x, HIST5-2AX, Histone H2A.X |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16104 |
| Иммуноген | Synthesized peptide derived from internal of human Histone H2AX. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF,WB:1:500-1:3000, IF:1:100-1:500 |
| Примечание | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.<br/><br/>The MGC Project Team, Genome Res. 14:2121-2127(2004).<br/>Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).<br/>Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody.
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Immunofluorescence analysis of COS7 cells, using Histone H2AX antibody.
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Наименование: Histone H2AX Antibody.