| Объем | 100 мкг |
| Синонимы | HLA class I histocompatibility antigen, alpha chain G (HLA G antigen) (MHC class I antigen G), HLA-G, HLA-6.0 HLAG |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P17693 |
| Иммуноген | Recombinant Human HLA class I histocompatibility antigen, alpha chain G protein (25-337AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HLA class I histocompatibility antigen, alpha chain G antibody at 2µg/ml + U251 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
 |
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA02109A0RB at dilution of 1:50
 |
Immunofluorescent analysis of 293T cells using CSB-PA02109A0RB at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: HLA-G antibody at 8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
 |
Immunofluorescence staining of Hela cells with CSB-PA02109A0RB at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
 |
| | |
Наименование: HLA-G Antibody.