| Объем | 50 мкг |
| Синонимы | 3-hydroxyacyl-CoA dehydrogenase type-2 (EC 1.1.1.35) (17-beta-hydroxysteroid dehydrogenase 10) (17-beta-HSD 10) (EC 1.1.1.51) (2-methyl-3-hydroxybutyryl-CoA dehydrogenase) (MHBD) (3-hydroxy-2-methylbutyryl-CoA dehydrogenase) (EC 1.1.1.178) (3-hydroxyacyl-CoA dehydrogenase type II) (Endoplasmic reticulum-associated amyloid beta-peptide-binding protein) (Mitochondrial ribonuclease P protein 2) (Mitochondrial RNase P protein 2) (Short chain dehydrogenase/reductase family 5C member 1) (Short-chain type dehydrogenase/reductase XH98G2) (Type II HADH), HSD17B10, ERAB HADH2 MRPP2 SCHAD SDR5C1 XH98G2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q99714 |
| Иммуноген | Recombinant Human 3-hydroxyacyl-CoA dehydrogenase type-2 protein (2-261AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Tags & Cell Markers, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: 3-hydroxyacyl-CoA dehydrogenase type-2 antibody at 2µg/ml
Lane 1: Mouse brain tissue
Lane 2: 293T whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Hela whole cell lysate
Lane 5: HT29 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 27, 26 kDa
Observed band size: 27 kDa
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Western Blot
Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate
All lanes: HSD17B10 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 26 kDa
Observed band size: 27 kDa
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Immunofluorescence staining of A549 cells with CSB-PA860776HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating HSD17B10 in 293T whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA860776HA01HU in 293T whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA860776HA01HU (8µg) + 293T whole cell lysate (500µg)
Lane 3: 293T whole cell lysate (10µg)
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Наименование: HSD17B10 Antibody.