Объем | 50 мкг |
Синонимы | Iroquois-class homeodomain protein IRX-4 (Homeodomain protein IRXA3) (Iroquois homeobox protein 4), IRX4, IRXA3 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P78413 |
Иммуноген | Recombinant Human Iroquois-class homeodomain protein IRX-4 protein (287-363AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200 |
Примечание | Likely to be an important mediator of ventricular differentiation during cardiac development. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Iroquois-class homeodomain protein IRX-4 |
Области исследований | Others |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Colo320 whole cell lysate
All lanes: IRX4 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 58 kDa
Observed band size: 55 kDa
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Immunofluorescence staining of MCF-7 cells with CSB-PA011836LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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