UGT1A1 AntibodyСпецификация| Объем | 50 мкг | | Синонимы | UDP-glucuronosyltransferase 1-1 (UDPGT 1-1) (UGT1~X1) (UGT1-01) (UGT1.1) (EC 2.4.1.17) (Bilirubin-specific UDPGT isozyme 1) (hUG-BR1) (UDP-glucuronosyltransferase 1-A) (UGT-1A) (UGT1A) (UDP-glucuronosyltransferase 1A1), UGT1A1, GNT1 UGT1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P22309 | | Иммуноген | Recombinant Human UDP-glucuronosyltransferase 1-1 protein (395-482AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 | | Примечание | UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX-alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4-methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | UDP-glucuronosyltransferase 1-1 | | Области исследований | Metabolism | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, PC-3 whole cell lysate, Rat liver tissue, Mouse liver tissue
All lanes: UGT1A1 antibody at 4.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 50 kDa
Observed band size: 60 kDa
 | IHC image of CSB-PA025570LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of HepG2 cells with CSB-PA025570LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: UGT1A1 Antibody.