| Объем | 50 мкг |
| Синонимы | Protein Wnt-3a, WNT3A |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P56704 |
| Иммуноген | Recombinant Human Protein Wnt-3a protein (19-351AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500 |
| Примечание | Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Developmental biology, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded Mouse heart tissue using CSB-PA15549A0Rb at dilution of 1: 50
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA15549A0Rb at dilution of 1:50
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA15549A0Rb at dilution of 1:50
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Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: WNT3A antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 43 kDa
Observed band size: 40 kDa
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IHC image of CSB-PA15549A0Rb diluted at 1:260 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: WNT3A Antibody.