| Объем | 50 мкг |
| Синонимы | X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Lupus Ku autoantigen protein p70) (Ku70) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 6), XRCC6, G22P1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P12956 |
| Иммуноген | Recombinant Human X-ray repair cross-complementing protein 6 ptotein (2-609AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: X-ray repair cross-complementing protein 6 antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 70, 66 kDa
Observed band size: 70 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate
All lanes: XRCC6 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70, 66 kDa
Observed band size: 70 kDa
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Immunohistochemistry of paraffin-embedded Mouse liver tissue using CSB-PA01617A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA01617A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA01617A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA01617A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA01617A0Rb at dilution of 1:100
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Immunofluorescent analysis of PC-3 cells using CSB-PA01617A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: XRCC6 Antibody.