| Объем | 100 мкл |
| Синонимы | 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P43403 |
| Иммуноген | Peptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 |
| Примечание | ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions<br/><br/>Chan AC, et al. (1995) EMBO J, 14(11): 2499-2508<br/>Kong G, et al. (1996) Mol Cell Biol, 16(9): 5026-5035<br/>Salomon AR, et al. (2003)Proc Natl Acad Sci U S A, 100(2): 443-448<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Области исследований | Tags & Cell Markers,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HL60 cells using Zap-70(Ab-493) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Ab-493) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: ZAP70 (Ab-493) Antibody.