| Объем | 50 мкг |
| Синонимы | Keratin, type I cytoskeletal 18 (Cell proliferation-inducing gene 46 protein) (Cytokeratin-18) (CK-18) (Keratin-18) (K18), KRT18, CYK18 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P05783 |
| Иммуноген | Recombinant Human Keratin, type I cytoskeletal 18 protein (102-396AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Zebrafish |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:50-1:500 |
| Примечание | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: KRT18 antibody at 2µg/ml
Lane 1: K562 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Western blot
All lanes: KRT18 antibody at 12µg/ml
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
 |
Western Blot
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate
All lanes: KRT18 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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IHC image of CSB-PA10629A0Rb diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescent analysis of HepG2 cells using CSB-PA10629A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunocytochemistry analysis of human placenta tissue using CSB-PA10629A0Rb at dilution of 1:100
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Наименование: KRT18 Antibody.