Объем | 50 мкл |
Синонимы | Leukocyte immunoglobulin-like receptor subfamily B member 2 (LIR-2) (Leukocyte immunoglobulin-like receptor 2) (CD85 antigen-like family member D) (Immunoglobulin-like transcript 4) (ILT-4) (Monocyte/macrophage immunoglobulin-like receptor 10) (MIR-10) (CD antigen CD85d), LILRB2, ILT4 LIR2 MIR10 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8N423 |
Иммуноген | Recombinant Human Leukocyte immunoglobulin-like receptor subfamily B member 2 protein (406-563AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
Примечание | Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Leukocyte immunoglobulin-like receptor subfamily B member 2 |
Области исследований | Immunology |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293T whole cell lysate, Jurkat whole cell lysate
All lanes: LILRB2 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 65, 56, 52 kDa
Observed band size: 66 kDa
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Immunofluorescence staining of HepG2 cells with CSB-PA013000LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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