Объем | 50 мкг |
Синонимы | Lipoprotein lipase (LPL) (EC 3.1.1.34), LPL, LIPD |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P06858 |
Иммуноген | Recombinant Human Lipoprotein lipase protein (162-246AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:500-1:1000, IF:1:200-1:500 |
Примечание | The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL) (PubMed:27578112). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Lipoprotein lipase |
Области исследований | Cancer, Cardiovascular, Metabolism, Signal transduction, Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA013065LA01HU diluted at 1:800 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/4/CSB-PA013065LA01HU-IHC-1.jpg) |
Immunofluorescence staining of HepG2 cells with CSB-PA013065LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
![](http://www.cusabio.cn/images/antibody/4/CSB-PA013065LA01HU-IF.jpg) |
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