MAP3K8 (Ab-400) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | C-COT, cancer Osaka thyroid oncogene, COT proto-oncogene serine/threonine-protein kinase, EC 2.7.11.25, kinase Cot | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P41279 | | Иммуноген | Synthesized non-phosphopeptide derived from human MAP3K8 around the phosphorylation site of serine 400 (C-Q-S(p)-L-D). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.<br/><br/>Miyoshi J.,Mol. Cell. Biol. 11:4088-4096(1991).<br/>Aoki M., J. Biol. Chem. 268:22723-22732(1993).<br/>Chan A.M.,Oncogene 8:1329-1333(1993).<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using MAP3K8 (Ab-400) antibody.
 | Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K8 (Ab-400) antibody.
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Наименование: MAP3K8 (Ab-400) Antibody.