MCTS1 AntibodyСпецификация| Объем | 50 мкг | | Синонимы | Malignant T-cell-amplified sequence 1 (MCT-1) (Multiple copies T-cell malignancies), MCTS1, MCT1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9ULC4 | | Иммуноген | Recombinant Human Malignant T-cell-amplified sequence 1 protein (1-181AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:200-1:500 | | Примечание | Anti-oncogene that play a role in cell cycle regulation, decreases cell doubling time and anchorage-dependent growth, shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Involved in translation initiation, promotes recruitment of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role as translation enhancer, recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile, up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway, increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when overexpressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell, Positively regulates phosphorylation of MAPK1 and MAPK3. Involved in translation initiation, promotes aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | Malignant T-cell-amplified sequence 1 | | Области исследований | Cancer | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA892143HA01HU at dilution of 1:100
 | Immunofluorescence staining of PC-3 cells with CSB-PA892143HA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: MCTS1 Antibody.