| Объем | 100 мкл |
| Синонимы | NED8, NEDD-8, Ubiquitin-like protein NEDD8, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q15843 |
| Иммуноген | Synthesized peptide derived from Internal of human NEDD8. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 |
| Примечание | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.<br/><br/>Margaret A. Read, Mol. Cell. Biol., Apr 2000, 20: 2326 - 2333. <br/>Kenneth Wu, J. Biol. Chem., Aug 2003, 278: 28882 - 28891. <br/>Katsumi Kito, J. Biol. Chem., Jun 2001, 276: 20603 - 20609. <br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using NEDD8 antibody.
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Western blot analysis of extracts from Jurkat cells, using NEDD8 antibody.
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Western blot analysis of extracts from HuvEc cells (Lane 2), JK cells (Lane 3) and cos-7 cells (Lane 4), using NEDD8 antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: NEDD8 Antibody.