NFKBIA (Ab-32/36) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | I-kappa-B-alpha, IKBA, NF-kappaB inhibitor alpha, NFKBI, NFKBIA | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P25963 | | Иммуноген | Peptide sequence around aa.30~34/34~38 (H-D-S-G-L- D-S -M-K) derived from Human IkB-a. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. <br/><br/>Mattioli I, et al. (2004)J Immunol, 172(10): 6336-44.<br/>Courtois G, et al. (2003)J Clin Invest, 112(7): 1108-15.<br/>Nair A, et al. (2003) Oncogene, 22(1): 50-8.<br/>Fan C, et al. (2002)J Cell Sci, 115(Pt 24): 4843-53.<br/>Schubert SY, et al. (2002)FASEB J, 16(14): 1931-3.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 and 3T3 cells using IkB-a(Ab-32/36) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Ab-32/36) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Ab-32/36) Antibody.
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Наименование: NFKBIA (Ab-32/36) Antibody.