Объем | 100 мкл |
Синонимы | Kinetochore protein Nuf2 (hNuf2) (hNuf2R) (hsNuf2) (Cell division cycle-associated protein 1), NUF2, CDCA1 NUF2R |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9BZD4 |
Иммуноген | Recombinant Human Kinetochore protein Nuf2 protein (165-464AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Kinetochore protein Nuf2 |
Области исследований | Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: NUF2 antibody at 5.29µg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA871623ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA871623ESR1HU at dilution of 1:100
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