| Объем | 100 мкг |
| Синонимы | Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P37198 |
| Иммуноген | Recombinant Human Nuclear pore glycoprotein p62 protein (173-522AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000 |
| Примечание | Essential component of the nuclear pore complex (PubMed:1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Nuclear pore glycoprotein p62 |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Tags & Cell Markers, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, RAW264.7 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, A549 whole cell lysate
All lanes: NUP62 antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 kDa
Observed band size: 62 kDa
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IHC image of CSB-PA016204LA01HU diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating NUP62 in A549 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA016204LA01HU in A549 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA016204LA01HU (6µg) + A549 whole cell lysate (1mg)
Lane 3: A549 whole cell lysate (20µg)
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Наименование: NUP62 Antibody.