| Объем | 100 мкг |
| Синонимы | Homeobox protein OTX2 (Orthodenticle homolog 2), OTX2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P32243 |
| Иммуноген | Recombinant Human Homeobox protein OTX2 protein (1-297AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Zebrafish |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Neuroscience, Developmental biology, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: OTX2 antibody at 4µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Mouse lung tissue
Lane 4: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32, 33 kDa
Observed band size: 32, 37, 43, 44, 62 kDa
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA017299LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA017299LA01HU at dilution of 1:100
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IHC image of CSB-PA017299LA01HU diluted at 1:250 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA017299LA01HU diluted at 1:250 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: OTX2 Antibody.