| Объем | 100 мкг |
| Синонимы | PHD finger protein 6 (PHD-like zinc finger protein), PHF6, CENP-31 KIAA1823 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q8IWS0 |
| Иммуноген | Recombinant Human PHD finger protein 6 protein (2-312AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | PHD finger protein 6 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: PHF6 antibody at 2µg/ml
Lane 1: K562 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42, 36, 38 kDa
Observed band size: 42, 85 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA017917LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA017917LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: PHF6 antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42, 36, 38 kDa
Observed band size: 42 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA017917LA01HU at dilution of 1:100
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Immunoprecipitating PHF6 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA017917LA01HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA017917LA01HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
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Immunofluorescence staining of Hela cells with CSB-PA017917LA01HU at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: PHF6 Antibody.