| Объем | 100 мкл |
| Синонимы | cyclin H, p34, p37, MO15-associated protein, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P51946 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from Human Cyclin H . |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.<br/><br/>Maekelae T.P., Nature 371:254-257(1994).<br />Fisher R.P., Cell 78:713-724(1994).<br />The MGC Project Team, Genome Res. 14:2121-2127(2004).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr315 |
| Области исследований | Epigenetics and Nuclear Signaling,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody. The lane on the left is treated with antigen-specific peptide.
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Наименование: Phospho-CCNH (Thr315) Antibody.