| Объем | 100 мкл |
| Синонимы | CDC25M1, MPIP3, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P30307 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.<br/><br/>Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348.<br/>Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860.<br/>Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677.<br/>Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995. <br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser216 |
| Области исследований | Epigenetics and Nuclear Signaling,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated or treated with serum using cdc25C(Phospho-Ser216) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C(Phospho-Ser216) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using cdc25C(Phospho-Ser216) Antibody.
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Наименование: Phospho-CDC25C (Ser216) Antibody.