| Объем | 100 мкл |
| Синонимы | CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P46527 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.<br/><br/>Eguchi H, et al. (2003) Cancer Res, 63(15): 4739-46<br/>Le XF, et al. (2003) J Biol Chem,<br/>Connor MK, et al. (2003) |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr187 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN-a using p27Kip1(Phospho-Thr187) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1(Phospho-Thr187) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-CDKN1B (Thr187) Antibody.