| Объем | 100 мкл |
| Синонимы | ECK, MPK-5, SEK2, kinase EphA2, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P29317/P29320/P54764 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y(p)-V-R)/(R-T-Y(p)-V-D) derived from Human EPHA2/3/4. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
| Примечание | <p>Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. <br/><br/>Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).<br />Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).<br />Zhuang G., J. Biol. Chem. 282:2683-2694(2007)<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr588/596 |
| Области исследований | Neuroscience,Cardiovascular |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody.
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Наименование: Phospho-EPHA2/EPHA3/EPHA4 (Tyr588/596) Antibody.