| Объем | 100 мкл |
| Синонимы | HD5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UQL6 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 498 (T-Q-S(p)-S-P) derived from Human HDAC5/7. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. <br /><br />Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019. <br />McKinsey TA, et al. (2000) Nature. 408(6808): 106-111. <br /> ,</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser498 |
| Области исследований | Epigenetics and Nuclear Signaling,Cardiovascular,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells untreated or treated with serum starvation using HDAC5(Phospho-Ser498) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5(Phospho-Ser498) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-HDAC5 (Ser498) Antibody.