| Объем | 100 мкл |
| Синонимы | Histone H1.5 (Histone H1a) (Histone H1b) (Histone H1s-3), HIST1H1B, H1F5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16401 |
| Иммуноген | Peptide sequence around site of phospho-Ser (17) derived from Human Histone H1.5. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, ICC, IF, Recommended dilution: WB:1:200-1:2000, ICC:1:20-1:200, IF:1:20-1:200 |
| Примечание | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H1.5 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: HIST1H1B antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 kDa
Observed band size: 32 kDa
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Immunocytochemistry analysis of HepG2 cells using CSB-PA010377PA17phHU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA010377PA17phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: Phospho-HIST1H1B (S17) Antibody.