| Объем | 100 мкл |
| Синонимы | MERL, NF2, Neurofibromin 2, SCH, Schwannomerlin |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P35240 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
| Примечание | <p>Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. <br /><br />Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol , 25: 2384 - 2394. <br />Hi-Su Yang, et al. (2006) Cancer Res , 66: 2708 - 2715. <br />R Bohni, et al. (1994) J. Biol. Chem , 269: 14541 - 14545. <br />Adam J. Ratner, et al. (2001) J. Biol. Chem , 276: 19267 - 19275.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser518 |
| Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) using Merlin(Phospho-Ser518) Antibody.
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Immunofluorescence staining of methanol-fixed Hela cells using Merlin(Phospho-Ser518) Antibody.
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Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) Antibody.
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Наименование: Phospho-NF2 (Ser518) Antibody.