| Объем | 100 мкл |
| Синонимы | CADTK, FADK 2, FAK2, PTK2B, RAFTK |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14289 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 579(E-D-Y(p)-Y-K) derived from Human PYK2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.<br/><br/>Lev S., Nature 376:737-745(1995).<br />Herzog H., Genomics 32:484-486(1996).<br />Sasaki H., J. Biol. Chem. 270:21206-21219(1995).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr579 |
| Области исследований | Cancer,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-PTK2B (Tyr579) Antibody.