Объем | 50 мкг |
Синонимы | DNA-directed RNA polymerase II subunit RPB3 (RNA polymerase II subunit 3) (RNA polymerase II subunit B3) (DNA-directed RNA polymerase II 33 kDa polypeptide) (RPB33) (DNA-directed RNA polymerase II subunit C) (RPB31), POLR2C |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P19387 |
Иммуноген | Recombinant Human DNA-directed RNA polymerase II subunit RPB3 protein (2-275AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
Примечание | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
All lanes: POLR2C antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA018329LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA018329LA01HU at dilution of 1:100
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