Объем | 100 мкл |
Синонимы | cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1, TSE1, PRKAR1A, PKR1, PRKAR1, TSE1 |
Тип антител | Recombinant Antibody |
Species | Human |
UniProt ID | P10644 |
Иммуноген | A synthesized peptide derived from human PRKAR1A |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
Клональность | Monoclonal |
Изотип | Rabbit IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Affinity-chromatography |
Области исследований | Signal Transduction |
Аббревиатура | cAMP-dependent protein kinase type I-alpha regulatory subunit |
Примечание | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: U87 whole cell lysate
All lanes: PRKAR1A antibody at 1.1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 39 KDa
Observed band size: 43 KDa
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Immunofluorescence staining of Hela cells with CSB-RA018694A0HU at 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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