Объем | 100 мкл |
Синонимы | Polypyrimidine tract-binding protein 3 (Regulator of differentiation 1) (Rod1), PTBP3, ROD1 |
Клональность | Polyclonal |
Организм | Human |
uniprot | O95758 |
Иммуноген | Recombinant Human Polypyrimidine tract-binding protein 3 protein (37-55AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Polypyrimidine tract-binding protein 3 |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate
All lanes: PTBP3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 57, 61, 50 kDa
Observed band size: 60 kDa
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IHC image of CSB-PA020060NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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