Объем | 100 мкг |
Синонимы | E3 ubiquitin-protein ligase RNF43 (EC 2.3.2.27) (RING finger protein 43) (RING-type E3 ubiquitin transferase RNF43), RNF43 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q68DV7 |
Иммуноген | Recombinant Human E3 ubiquitin-protein ligase RNF43 protein (566-697AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IHC, Recommended dilution: IHC:1:500-1:1000 |
Примечание | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | E3 ubiquitin-protein ligase RNF43 |
Области исследований | Cancer, Cell biology, Immunology |
Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA019892LA01HU diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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