RUNX1 (Ab-435) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Acute myeloid leukemia 1 protein, CBF-alpha 2, CBFA2, Core-binding factor, alpha 2 subunit | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q01196 | | Иммуноген | Synthesized non-phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-S(p)-P-T). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.<br/><br/>Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).<br/>Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).<br/>Nucifora G., Blood 81:2728-2734(1993).<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Developmental biology,Stem cells | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Ab-435) antibody.
 | Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using AML1 (Ab-435) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: RUNX1 (Ab-435) Antibody.