Объем | 50 мкг |
Синонимы | Sphingosine 1-phosphate receptor 2 (S1P receptor 2) (S1P2) (Endothelial differentiation G-protein coupled receptor 5) (Sphingosine 1-phosphate receptor Edg-5) (S1P receptor Edg-5), S1PR2, EDG5 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O95136 |
Иммуноген | Recombinant Human Sphingosine 1-phosphate receptor 2 protein (293-353AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:200-1:500 |
Примечание | Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Sphingosine 1-phosphate receptor 2 |
Области исследований | Epigenetics and Nuclear Signaling, Neuroscience, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: S1PR2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
![](http://www.cusabio.cn/images/antibody/6/CSB-PA020651LA01HU-WB.jpg) |
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA020651LA01HU at dilution of 1:100
![](http://www.cusabio.cn/images/antibody/4/CSB-PA020651LA01HU-IHC-1.jpg) |
Immunofluorescence staining of SH-SY5Y cells with CSB-PA020651LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
![](http://www.cusabio.cn/images/antibody/5/CSB-PA020651LA01HU-IF.jpg) |
| |