SMAD3 (Ab-204) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P84022 | | Иммуноген | Synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204 (A-G-S(p)-P-N). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 | | Примечание | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.<br/><br/>Zhang Y., Nature 383:168-172(1996).<br/>Riggins G.J., Nat. Genet. 13:347-349(1996).<br/>Arai T., Cancer Lett. 122:157-163(1998).<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Metabolism,Signal transduction,Stem cells | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HUVEC cells, using Smad3 (Ab-204) antibody.
 | Immunohistochemistry analysis of paraffin-embedded human colon colon tissue using Smad3 (Ab-204) antibody.
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Наименование: SMAD3 (Ab-204) Antibody.