SPARC AntibodyСпецификацияОбъем | 100 мкл | Синонимы | ON, BM-40, | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P09486 | Иммуноген | Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC. | Источник | Rabbit | Видовая специфичность | Human | Применение | ELISA, WB,WB:1:500-1:1000 | Примечание | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites, an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.<br/><br/>Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)<br/>Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988) <br/> Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995) | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | Форма | liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | Области исследований | Cancer,Cardiovascular,Cell biology,Developmental biology,Signal transduction,Stem cells | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extract from T98G cells using SPARC Antibody
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