Объем | 50 мкг |
Синонимы | Protein Spindly (hSpindly) (Arsenite-related gene 1 protein) (Coiled-coil domain-containing protein 99) (Rhabdomyosarcoma antigen MU-RMS-40.4A) (Spindle apparatus coiled-coil domain-containing protein 1), SPDL1, CCDC99 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q96EA4 |
Иммуноген | Recombinant Human Protein Spindly protein (1-300AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: SPDL1 antibody at 4µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 71, 59, 62 kDa
Observed band size: 71 kDa
|
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA842662LA01HU at dilution of 1:100
|
| |