| Объем | 50 мкг |
| Синонимы | Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)], Tgfb1 |
| Клональность | Polyclonal Antibody |
| Организм | Mouse |
| uniprot | P04202 |
| Иммуноген | Recombinant Mouse Transforming growth factor beta-1 protein (30-278AA) |
| Источник | Rabbit |
| Видовая специфичность | Mouse, Human |
| Применение | ELISA, WB, IP, Recommended dilution: WB:1:500-1:5000, IP:1:200-1:2000 |
| Примечание | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed:18368049). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Transforming growth factor beta-1 |
| Области исследований | Cancer, Cardiovascular, Metabolism, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: Tgfb1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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Immunoprecipitating Tgfb1 in MCF-7 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA023446LA01MO in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA023446LA01MO (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (10µg)
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Наименование: Tgfb1 Antibody.