| Объем | 100 мкл |
| Синонимы | Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, tumor protein p53 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04637 |
| Иммуноген | Synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 378 (S-T-S(p)-R-H). |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB,WB:1:500-1:3000 |
| Примечание | Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediatedapoptosis. 7 0 2 5<br/><br/>Zakut-Houri R., EMBO J. 4:1251-1255(1985).<br/>Lamb P., Mol. Cell. Biol. 6:1379-1385(1986).<br/>Harlow E., Mol. Cell. Biol. 5:1601-1610(1985).<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells, using p53 (Ab-378) antibody.
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Western blot analysis of extracts from HepG2 cells (Lane 2), using p53 (Ab-378) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: TP53 (Ab-378) Antibody.