UBC AntibodyСпецификация| Объем | 50 мкл | | Синонимы | Polyubiquitin-C [Cleaved into: Ubiquitin], UBC | | Клональность | Polyclonal | | Организм | Human | | uniprot | P0CG48 | | Иммуноген | Recombinant Human Polyubiquitin-C protein (19-36AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, Recommended dilution: WB:1:1000-1:5000 | | Примечание | Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Абревеатура | Polyubiquitin-C | | Области исследований | Epigenetics and Nuclear Signaling, Neuroscience, Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, LO2 whole cell lysate, Jurkat whole cell lysate
All lanes: UBC antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
 | | | |
Информация для заказа |
Наименование: UBC Antibody.