| Объем | 50 мкл |
| Синонимы | 14-3-3 protein theta (14-3-3 protein T-cell) (14-3-3 protein tau) (Protein HS1), YWHAQ |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P27348 |
| Иммуноген | Recombinant Human 14-3-3 protein theta protein (1-245AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:500, IF:1:50-1:200 |
| Примечание | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Neuroscience, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: 14-3-3 protein theta antibody at 4µg/ml
Lane 1: A549 whole cell lysate
Lane 2: Mouse lung tissue
Lane 3: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA026290ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA026290ESR1HU at dilution of 1:100
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IHC image of CSB-PA026290ESR1HU diluted at 1:221 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA026290ESR1HU at 1:73, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: YWHAQ Antibody.