| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IF, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P14635 |
| Calculated MW | 55 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor(MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. |
| Immunogen | cyclin B1 |
| Synonyms | CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1 |
| Recommended dilution | WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 |
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab02122(Cyclin B1 Antibody) at dilution of 1:50. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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HeLa cells were subjected to SDS PAGE followed by western blot with FNab02122(CCNB1 antibody) at dilution of 1:500
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