anti- CUL4A antibodyСпецификация| Clonality | monoclonal | | Host | Mouse | | Specificity | Human, Monkey, Mouse, Rat | | Tested Application | ELISA, WB, IHC, IP, IF | | Delivery Time | 2 to 4 working days | | Clone ID | 5A7 | | Isotype | IgG1 | | Form | liquid | | Purification | Protein A+G purification | | Purity | ≥95% as determined by SDS-PAGE | | Uniprot ID | Q13619 | | Gene ID | | | Ссылка на страницу на сайте производителя | ссылка | | Инструкция | PDF | | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) | | Background | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled(H3.1) and replication-independent(H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL. | | Immunogen | cullin 4A | Immunohistochemistry of paraffin-embedded human heart tissue slide using FNab02077(CUL4A Antibody) at dilution of 1:200
 | HeLa cells were subjected to SDS PAGE followed by western blot with FNab02077(CUL4A antibody) at dilution of 1:500
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Информация для заказа| Область использования: | Производство: | Fine Biotech | | Метод: | Антитела | | Объем: | 100µg | | Кат. номер: | FNab02077 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: anti- CUL4A antibody.
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