| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human |
| Tested Application | ELISA, WB, IP, IF |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P27695 |
| Gene ID | |
| Calculated MW | 39 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Background | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. |
| Immunogen | APEX nuclease (multifunctional DNA repair enzyme) 1 |
| Synonyms | APE, APE1, APEX, APX, HAP1, REF1 |
| Recommended dilution | WB: 1:500 - 1:2000; IF: 1:50 - 1:100; IP: 1:50 - 1:200 |
Immunofluorescence analysis of HeLa cells using FNab00479(APEX1 antibody) at dilution of 1:50
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HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00479(APEX1 antibody) at dilution of 1:1000
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