anti- GFP tag antibody

Спецификация

Highlight	Pubmed/Publication
Clonality	monoclonal
Host	Mouse
Specificity	Recombinant protein, Human
Tested Application	ELISA, WB, IP, IF
Delivery Time	2 to 4 working days
Clone ID	0D8
Isotype	IgG1
Form	liquid
Purification	Protein A+G purification
Purity	≥95% as determined by SDS-PAGE
Calculated MW	30 kDa
Ссылка на страницу на сайте производителя	ссылка
Инструкция	PDF
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background	Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.
Immunogen	GFP tag
Synonyms	CFP, eGFP, eYFP, GFP, GFP tag, YFP
Recommended dilution	WB: 1:2000-1:16000; IP: 1:1000-1:4000; IF: 1:20-1:200
eGFP-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03434(GFP antibody) at dilution of 1:10000

Информация для заказа

Область использования: Моноклональные антитела	Производство:	Fine Biotech
	Метод:	Антитела
	Объем:	100µg
	Кат. номер:	FNab03434
Цена (с НДС 20%):	по запросу	В корзину
Наименование: anti- GFP tag antibody. Примечание: