Clonality | polyclonal |
Host | Rabbit |
Specificity | Human, Rat |
Tested Application | ELISA, WB, IHC, IF, IP |
Delivery Time | 2 to 4 working days |
Isotype | IgG |
Form | liquid |
Purification | Immunogen affinity purified |
Purity | ≥95% as determined by SDS-PAGE |
Uniprot ID | Q13084 |
Gene ID | |
Calculated MW | 30 kDa |
Ссылка на страницу на сайте производителя | ссылка |
Инструкция | PDF |
Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. |
Immunogen | mitochondrial ribosomal protein L28 |
Synonyms | MAAT1 |
Recommended dilution | WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100; IP: 1:50 - 1:200 |
Immunohistochemistry of paraffin-embedded human gastric cancer using FNab05325(MRPL28 antibody) at dilution of 1:50
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A375 cells were subjected to SDS PAGE followed by western blot with FNab05325(MRPL28 antibody) at dilution of 1:1000
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