Clonality | polyclonal |
Host | Rabbit |
Specificity | Recombinant protein, Mouse |
Tested Application | ELISA, WB, IF |
Delivery Time | 2 to 4 working days |
Isotype | IgG |
Form | liquid |
Purification | Immunogen affinity purified |
Purity | ≥95% as determined by SDS-PAGE |
Ссылка на страницу на сайте производителя | ссылка |
Инструкция | PDF |
Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
Background | Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is a rabbit polyclonal antibody raised against 3xFlag(3xDYKDDDDKT) sequence and recognizes the(3x)DYKDDDDK peptide and detects FLAG-tagged proteins. |
Immunogen | Flag tag |
Recommended dilution | WB: 1:1000-1:4000; IF: 1:10-1:100 |
Flagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03154(Flag tag antibody) at dilution of 1:1000
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