anti- P27; KIP1 antibodyСпецификация| Clonality | polyclonal | | Host | Rabbit | | Specificity | Human, Mouse, Rat | | Tested Application | ELISA, WB, IP, IHC, IF | | Delivery Time | 2 to 4 working days | | Isotype | IgG | | Form | liquid | | Purification | Immunogen affinity purified | | Purity | ≥95% as determined by SDS-PAGE | | Uniprot ID | P46527 | | Gene ID | | | Calculated MW | 21 kDa | | Ссылка на страницу на сайте производителя | ссылка | | Инструкция | PDF | | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) | | Background | CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer. | | Immunogen | cyclin-dependent kinase inhibitor 1B(p27, Kip1) | | Synonyms | CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1 | | Recommended dilution | WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500 | Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06068(P27; KIP1 Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
 | NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab06068(P27; KIP1 Antibody) at dilution of 1:600
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Информация для заказа| Область использования: | Производство: | Fine Biotech | | Метод: | Антитела | | Объем: | 100µg | | Кат. номер: | FNab06068 | | Цена (с НДС 20%): | по запросу | В корзину  | Наименование: anti- P27; KIP1 antibody.
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