| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, IHC, WB, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | Q15118 |
| Gene ID | |
| Calculated MW | 50kd |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. |
| Immunogen | pyruvate dehydrogenase kinase, isozyme 1 |
| Synonyms | PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1 |
| Recommended dilution | WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500 |
Immunohistochemistry of paraffin-embedded human liver using FNab06275(PDK1 antibody) at dilution of 1:100
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mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab06275(PDK1 Antibody) at dilution of 1:1000
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