| Clonality | polyclonal |
| Host | Rabbit |
| Specificity | Human, Mouse, Rat |
| Tested Application | ELISA, WB, IP |
| Delivery Time | 2 to 4 working days |
| Isotype | IgG |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Uniprot ID | P45974 |
| Gene ID | |
| Calculated MW | 73 kDa,Observed 55 kDa,73 kDa |
| Ссылка на страницу на сайте производителя | ссылка |
| Инструкция | PDF |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Background | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. |
| Immunogen | ubiquitin specific peptidase 5(isopeptidase T) |
| Synonyms | Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5 |
| Recommended dilution | WB: 1:500-1:5000; IP: 1：500-1：5000 |
A549 cells were subjected to SDS PAGE followed by western blot with FNab09339(USP5 antibody) at dilution of 1:1000
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Наименование: anti- USP5 antibody.